a user's guide Pryzyborski, Stefan editor text xx Chichester, West Sussex, UK Wiley/Blackwell 2017 monographic und

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xvii, 406 pages: color illustration 25 cm. Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs.-Offers a comprehensive, focused guide to the current state-of-the-art technologies available for 3D cell culture -Features contributions from leading developers and researchers active in 3D cell technology -Gives clear instruction and guidance on performing specific 3D culture methods, along with colour illustrations and examples of where such technologies have been successfully applied -Includes information on resources and technical support to help initiate the use of 3D culture methods. Title Page ; Copyright Page; Contents; List of contributors; Preface; List of abbreviations; Chapter 1 An introduction to the third dimension for routine cell culture ; Introduction; Aggregate-based technologies ; Scaffold-based technologies ; 3D bioreactors; Barriers to adoption and future directions; References; Part I Aggregate-based technologies ; Chapter 2 Gravity-enforced microtissue engineering ; Introduction; Product description; Tumour microtissue size profiling for drug testing; Conclusions; Troubleshooting and notes (Table 2.3); Summary; References. Chapter 3 Physiologically relevant spheroid models for three-dimensional cell culture Introduction; Description of technology; Experimental results obtained using the Perfecta3D hanging drop plates; Troubleshooting; Conclusion; References; Chapter 4 NanoCulture Plate: A scaffold-based high-throughput three-dimensional cell culture system suitable for live imaging and co-culture ; Introduction; Description of technology; Example of application; Troubleshooting; Summary; References; Chapter 5 Micro-moulded non-adhesive hydrogels to form multicellular microtissues -- the 3D Petri Dish® IntroductionDescription of technology; Applications; Protocols; Troubleshooting; Conclusion; References; Chapter 6 Organotypic microtissues on an air-liquid interface ; Introduction; Description of technology; 3D neural OrganDOT" tissues for neurotoxicology studies; Troubleshooting; References; Part II Hydrogels; Chapter 7 Materials and assay systems used for three?dimensional cell culture; Introduction; Description of technology; Applications of 3D cultures in vitro; Conclusion; References; Chapter 8 HyStem®, a customisable hyaluronan?based hydrogel matrix for 3D cell culture; Introduction. Description of technologyApplications; Troubleshooting; Conclusion; Appendix; References; Chapter 9 3-D Life biomimetic hydrogels: A modular system for cell environment design ; Introduction; Applications; Description of technology; A tumour-stroma model: 3D co-culture of MCF-7 cells and primary fibroblasts ; Troubleshooting; Conclusion; References; Part III Scaffolds; Chapter 10 Alvetex®, a highly porous polystyrene scaffold for routine three-dimensional cell culture ; Introduction; Description of technology; Example applications of Alvetex Scaffold technology; Troubleshooting; Conclusion.; Includes index. edited by Stefan Przyborksi. in English Cell culture Cytology Technique Cell culture techniques Method 571.638 T22u c2017 9781118851500 251212 20260803094212.0