000 04141nam a2200349Ia 4500
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008 251212s2017 xx 000 0 und d
245 1 _aTechnology platforms for 3D cell structure:
_ba user's guide /
_cedited by Stefan Przyborksi.
700 0 _aPryzyborski, Stefan,
_eeditor
260 _aChichester, West Sussex, UK :
_bWiley/Blackwell,
_c2017.
300 _axvii, 406 pages:
_bcolor illustration
300 _c25 cm.
020 _a9781118851500
650 _aCell culture
650 _aCytology
_xTechnique
650 _aCell culture techniques
_xMethod
944 _aREF
050 _a571.638 T22u c2017
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944 _b1
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953 _pSH0251
546 _ain English
953 _cSenior High School Library
505 _aTitle Page ; Copyright Page; Contents; List of contributors; Preface; List of abbreviations; Chapter 1 An introduction to the third dimension for routine cell culture ; Introduction; Aggregate-based technologies ; Scaffold-based technologies ; 3D bioreactors; Barriers to adoption and future directions; References; Part I Aggregate-based technologies ; Chapter 2 Gravity-enforced microtissue engineering ; Introduction; Product description; Tumour microtissue size profiling for drug testing; Conclusions; Troubleshooting and notes (Table 2.3); Summary; References. Chapter 3 Physiologically relevant spheroid models for three-dimensional cell culture Introduction; Description of technology; Experimental results obtained using the Perfecta3D hanging drop plates; Troubleshooting; Conclusion; References; Chapter 4 NanoCulture Plate: A scaffold-based high-throughput three-dimensional cell culture system suitable for live imaging and co-culture ; Introduction; Description of technology; Example of application; Troubleshooting; Summary; References; Chapter 5 Micro-moulded non-adhesive hydrogels to form multicellular microtissues -- the 3D Petri Dish® IntroductionDescription of technology; Applications; Protocols; Troubleshooting; Conclusion; References; Chapter 6 Organotypic microtissues on an air-liquid interface ; Introduction; Description of technology; 3D neural OrganDOT" tissues for neurotoxicology studies; Troubleshooting; References; Part II Hydrogels; Chapter 7 Materials and assay systems used for three?dimensional cell culture; Introduction; Description of technology; Applications of 3D cultures in vitro; Conclusion; References; Chapter 8 HyStem®, a customisable hyaluronan?based hydrogel matrix for 3D cell culture; Introduction. Description of technologyApplications; Troubleshooting; Conclusion; Appendix; References; Chapter 9 3-D Life biomimetic hydrogels: A modular system for cell environment design ; Introduction; Applications; Description of technology; A tumour-stroma model: 3D co-culture of MCF-7 cells and primary fibroblasts ; Troubleshooting; Conclusion; References; Part III Scaffolds; Chapter 10 Alvetex®, a highly porous polystyrene scaffold for routine three-dimensional cell culture ; Introduction; Description of technology; Example applications of Alvetex Scaffold technology; Troubleshooting; Conclusion.; Includes index.
520 _aTechnology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs.-Offers a comprehensive, focused guide to the current state-of-the-art technologies available for 3D cell culture -Features contributions from leading developers and researchers active in 3D cell technology -Gives clear instruction and guidance on performing specific 3D culture methods, along with colour illustrations and examples of where such technologies have been successfully applied -Includes information on resources and technical support to help initiate the use of 3D culture methods.
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336 _atext
337 _aunmediated
338 _avolume
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_cBK
_alisuga
_b2018-02-27 09:41:17
_dlisuga
_e2018-02-27 09:42:26
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